EPB41L5 重组蛋白, Human (E. coli, His)

EPB41L5 Protein, Human, Recombinant (E. coli, His)

产品编号 TMPH-00995

Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells. EPB41L5 Protein, Human, Recombinant (E. coli, His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 85.5 kDa and the accession number is Q9HCM4.

EPB41L5 Protein, Human, Recombinant (E. coli, His)

产品编号 TMPH-00995

规格	价格	库存
20 μg	¥ 1,800	20日内发货
100 μg	¥ 3,760	20日内发货
1 mg	¥ 16,000	20日内发货

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问如何设置母液浓度？

答母液浓度需低于官网给出的溶解度，在这个范围内，根据工作液浓度设置母液浓度，细胞实验中，建议母液浓度设定在工作液浓度的 1000 倍以上。

问如何稀释工作液？

答根据您的工作液浓度，计算母液所需要稀释的倍数和所需母液的体积。建议用水、生理盐水、PBS等溶剂进行稀释，如果是细胞实验，可以使用培养基。取适当的溶剂缓慢加入到母液中，直到达到您的工作液浓度，可以通过涡旋或者移液枪轻轻吹打帮助混匀。我们的化合物大部分是脂溶性的，所以在使用 PBS 或者培养基等稀释工作液的时候，会有沉淀析出，这些都是正常现象，大多时候可以通过超声完全溶解。工作液建议现配现用！

问抑制剂是否需要灭菌？

答如果您使用 DMSO 配制：不建议灭菌，DMSO 本身具有极强的杀菌力，配制好的溶液就是无菌溶液。如果您确实担心的，可以放置 4℃ 冰箱过夜放置即可；依然有顾虑的话，需用有机系专用滤膜过滤。如果您使用水配制：可以用 0.22 μm 滤膜过滤灭菌。

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答一般来说，如果有需要避光储存的分子，我们会选择棕色玻璃瓶发货的。

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产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells. EPB41L5 Protein, Human, Recombinant (E. coli, His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 85.5 kDa and the accession number is Q9HCM4.
种属	Human
表达系统	E. coli
标签	N-10xHis
蛋白编号	Q9HCM4
别名	KIAA1548,Erythrocyte membrane protein band 4.1-like 5,EPB41L5,Band 4.1-like protein 5
氨基酸序列	MLSFFRRTLGRRSMRKHAEKERLREAQRAATHIPAAGDSKSIITCRVSLLDGTDVSVDLPKKAKGQELFDQIMYHLDLIESDYFGLRFMDSAQVAHWLDGTKSIKKQVKIGSPYCLHLRVKFYSSEPNNLREELTRYLFVLQLKQDILSGKLDCPFDTAVQLAAYNLQAELGDYDLAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMHVVKARDGNDYSLGLTPTGVLVFEGDTKIGLFFWPKITRLDFKKNKLTLVVVEDDDQGKEQEHTFVFRLDHPKACKHLWKCAVEHHAFFRLRGPVQKSSHRSGFIRLGSRFRYSGKTEYQTTKTNKARRSTSFERRPSKRYSRRTLQMKACATKPEELSVHNNVSTQSNGSQQAWGMRSALPVSPSISSAPVPVEIENLPQSPGTDQHDRKCIPLNIDLLNSPDLLEATIGDVIGASDTMETSQALNDVNVATRLPGLGEPEVEYETLKDTSEKLKQLEMENSPLLSPRSNIDVNINSQEEVVKLTEKCLNNVIESPGLNVMRVPPDFKSNILKAQVEAVHKVTKEDSLLSHKNANVQDAATNSAVLNENNVPLPKESLETLMLITPADSGSVLKEATDELDALLASLTENLIDHTVAPQVSSTSMITPRWIVPQSGAMSNGLAGCEMLLTGKEGHGNKDGISLISPPAPFLVDAVTSSGPILAEEAVLKQKCLLTTEL
蛋白构建	1-733 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	85.5 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells.

EPB41L5 Protein, Human, Recombinant (E. coli, His)

EPB41L5 Protein, Human, Recombinant (E. coli, His)

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