DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. DNAM-1/CD226 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.9 kDa and the accession number is Q15762.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 1,990 | 5日内发货 | ||
500 μg | ¥ 8,000 | 5日内发货 | ||
1 mg | ¥ 13,300 | 5日内发货 |
生物活性 | 1. Immobilized Human DNAM-1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.24μg/ml determined by ELISA (QC Test). 2. Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22μM as determined in a SPR assay. |
产品描述 | DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. DNAM-1/CD226 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.9 kDa and the accession number is Q15762. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | Q15762 |
别名 | PTA1, TLiSA1, DNAM1, CD226, CD226 molecule |
蛋白构建 | Glu19-Asn247 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
分子量 | 28.9 kDa (predicted). Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
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DNAM-1/CD226 Protein, Human, Recombinant (His & Avi) PTA1 CD 226 TLiSA1 DNAM-1 DNAM1 CD226 PTA 1 CD226 molecule CD-226 PTA-1 DNAM 1 recombinant recombinant-proteins proteins protein