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Cystatin F/CST7 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.2 kDa and the accession number is O76096.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 820 | 6-8日内发货 | |
| 10 μg | ¥ 1,350 | 6-8日内发货 | |
| 20 μg | ¥ 2,630 | 5日内发货 |
| 生物活性 | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 产品描述 | Cystatin F/CST7 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.2 kDa and the accession number is O76096. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | Tag Free |
| 蛋白编号 | O76096 |
| 别名 | cystatin F (leukocystatin),CMAP |
| 蛋白构建 | The mature form of the human Cystatin F (NP_003641.3) (Met 1-His 145) with five amino acids (DDDDK) at the C-terminus was expressed and purified. Predicted N terminal: Gly 20 |
| 蛋白纯度 | > 95 % as determined by SDS-PAGE |
| 分子量 | 15.2 kDa (predicted); 25 kDa (reducing condition, due to glycosylation) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis. |