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Claudin-18 Protein-VLP, Cynomolgus, Recombinant (His)

产品编号 TMPH-02423

Claudin-18 Protein-VLP, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis (This tag can be tested only under denaturing conditions) tag. The predicted molecular weight is 28.7 kDa and the accession number is A0A2K5VV62.

Claudin-18 Protein-VLP, Cynomolgus, Recombinant (His)

Claudin-18 Protein-VLP, Cynomolgus, Recombinant (His)

产品编号 TMPH-02423
Claudin-18 Protein-VLP, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis (This tag can be tested only under denaturing conditions) tag. The predicted molecular weight is 28.7 kDa and the accession number is A0A2K5VV62.
规格价格库存数量
20 μg
¥ 4,930
20日内发货
100 μg
¥ 11,800
20日内发货
1 mg
¥ 67,300
20日内发货
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实验操作小课堂
常见问题解答
产品是否需要避光?
一般来说,如果有需要避光储存的分子,我们会选择棕色玻璃瓶发货的。
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
PEG300 可以用 PEG400 替代吗?
PEG300 具有适度的粘稠度,有利于药物的溶解和给药过程,同时也能够提供良好的耐受性,不会对动物造成不良影响。PEG400 可以替代 PEG300。 PEG600 不是很建议,因为熔点接近常温。
细胞实验中,抑制剂如何溶解?
用 DMSO 配置较高浓度母液,母液存于 -80℃,建议多分装几管,一周内使用的存于 4℃,避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释,细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%,则需要在做预试验的时候,先进行溶剂阴性对照实验,排除溶剂对细胞的影响。
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
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产品信息

生物活性
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/mL can bind Anti-CLDN18.2 recombinant Antibody, the EC 50 is 2.806-3.641 ng/mL.
产品描述
Claudin-18 Protein-VLP, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis (This tag can be tested only under denaturing conditions) tag. The predicted molecular weight is 28.7 kDa and the accession number is A0A2K5VV62.
种属
Cynomolgus
表达系统
HEK293 Cells
标签N-6xHis (This tag can be tested only under denaturing conditions)
蛋白编号A0A2K5VV62
别名
CLDN18
氨基酸序列
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKTYDGGAHTEDELQSYPSKHDYV
蛋白构建
1-261 aa
分子量28.7 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 6% Trehalose, pH 7.4
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

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