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CHRM1 Protein, Rat, Recombinant (His)

产品编号 TMPH-03338

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. CHRM1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 21.1 kDa and the accession number is P08482.

CHRM1 Protein, Rat, Recombinant (His)

CHRM1 Protein, Rat, Recombinant (His)

产品编号 TMPH-03338
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. CHRM1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 21.1 kDa and the accession number is P08482.
规格价格库存数量
20 μg
¥ 1,800
20日内发货
100 μg
¥ 3,760
20日内发货
1 mg
¥ 16,000
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
产品溶解需要超声吗?如果没有超声仪怎么办?
超声可以加快粉末的溶解速度,如果您的化合物溶解不了,建议您尝试超声。如果您没有超声仪,建议您选择较低母液浓度,或者较少粉末量,尝试溶解。
收到产品发现冰盒都化了,怎么办?
一般化合物粉末对温度不敏感,加冰盒是为了防止极端气温,如果冰盒融化不影响产品质量。
抑制剂母液的储存方式?
母液配置完成后,一般储存于 -80℃,可以存 1 年以上。建议多分装几管,避免反复冻融,常用的存于 4 度,可放一周以上。
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. CHRM1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 21.1 kDa and the accession number is P08482.
种属
Rat
表达系统
E. coli
标签N-6xHis
蛋白编号P08482
别名
Muscarinic acetylcholine receptor M1,Chrm-1,Chrm1
氨基酸序列
RIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDSEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKEKKAART
蛋白构建
210-366 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量21.1 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

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