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AQP2 Protein, Human, Recombinant (His)

产品编号 TMPH-03800AQP2 Protein, Human, Recombinant (His)

AQP2 Protein, Human, Recombinant (His) is expressed in in vitro E. coli expression system with N-10xHis. The accession number is P41181.

AQP2 Protein, Human, Recombinant (His)

AQP2 Protein, Human, Recombinant (His)

产品编号 TMPH-03800AQP2 Protein, Human, Recombinant (His)
AQP2 Protein, Human, Recombinant (His) is expressed in in vitro E. coli expression system with N-10xHis. The accession number is P41181.
规格价格库存数量
20 μg
¥ 9,580
20日内发货
100 μg
¥ 16,000
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
细胞实验中,抑制剂如何溶解?
用 DMSO 配置较高浓度母液,母液存于 -80℃,建议多分装几管,一周内使用的存于 4℃,避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释,细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%,则需要在做预试验的时候,先进行溶剂阴性对照实验,排除溶剂对细胞的影响。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
收到粉状试剂粘壁严重,如何处理?
建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
产品溶解需要超声吗?如果没有超声仪怎么办?
超声可以加快粉末的溶解速度,如果您的化合物溶解不了,建议您尝试超声。如果您没有超声仪,建议您选择较低母液浓度,或者较少粉末量,尝试溶解。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it.
产品描述
AQP2 Protein, Human, Recombinant (His) is expressed in in vitro E. coli expression system with N-10xHis. The accession number is P41181.
种属
Human
表达系统
in vitro E. coli expression system
标签N-10xHis
蛋白编号P41181
别名
WCH-CD,Water channel protein for renal collecting duct,Collecting duct water channel protein,Aquaporin-CD (AQP-CD),Aquaporin-2,AQP-2,AQP2,ADH water channel
氨基酸序列
MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGLGIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAGAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA
蛋白构建
1-271 aa
蛋白纯度
>85% as determined by SDS-PAGE.
分子量30.3 kDa (Predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

参考文献

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  • 复溶 计算器
  • 重组蛋白稀释 计算器
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