Vinculin Loading Control Antibody (5P889) is a Rabbit antibody targeting Vinculin. Vinculin Loading Control Antibody (5P889) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

5P889

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human HUVEC cell lysates
Primary: Anti-Vinculin (Loading Control) (TMAB-01968) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 135 kDa
2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Endometrium; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Mouse Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vinculin Monoclonal Antibody, Unconjugated (TMAB-01968) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

IF=1:500-2000; IHC-Fr=1:500-2000; IHC-P=1:500-2000; WB=1:1000-2000

Monoclonal

Recombinant Protein: Human Vinculin

Human

VCL

Vinculin

Cardiac arrhythmias,Actins,Actin Binding Proteins

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.