USP1-IN-4, at concentrations ranging from 0.0005 to 10 μM, significantly inhibits USP1/UAF1 activity with an IC50 of 2.44 nM following a 15-minute treatment [1]. Additionally, USP1-IN-4 impedes the growth of MDA-MB-436 cells over a 7-day period, also within the 0.0005 to 10 μM range, yielding an IC50 of 103.75 nM [1]. In a cell viability assay using the MDA-MB-436 cell line, a concentration range of 0.0005 to 10 μM was used, and cell growth inhibition was observed with an IC50 of 103.75 nM.