RB-3 decreases the global level of H2A ubiquitination and induces differentiation in leukemia cell lines and primary acute myeloid leukemia (AML) samples[1]. RB-3 inhibits H2A ubiquitination with an IC 50 of 1.6 μM[1]. RB-3 inhibits PRC1 and disrupts the interaction with nucleosomes[1]. RB-3 reduces colony formation in a mixed lineage leukemia (MLL)-eleven nineteen leukemia (ENL) model[1]. Western Blot Analysis[1]Cell Line: TEX cells. Concentration: 6, 12, 25 μM. Incubation Time: 8d. Result: Dose-dependently increased C/EBPα-p42 protein levels.