Rabbit Anti-Mouse IgG3 Secondary Antibody-HRP (7Z583) is a HRP-conjugated Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody-HRP (7Z583) can be used in WB,ELISA,IHC-P.

Monoclonal Rabbit IgG

7Z583

Mouse

1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG3 Secondary Antibody (HRP), Rabbit MAb reacts only to mouse IgG3. No cross reactivity with Mouse IgG1, IgG2a, IgG2b, IgM, Rabbit IgG, Human IgG. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.5 μg/mL of Rabbit Anti-Mouse IgG3 Secondary Antibody (HRP), Rabbit MAb was used as secondary antibody.

2. Immunohistochemical analysis of PFA-fixed paraffin-embedded human liver tissue sections, with VEGF primary antibody (1/500) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/6400) was used as the secondary antibody. (Validation Experiment)

3. Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KRT7 primary antibody (1/200) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/3200) was used as the secondary antibody. (Validation Experiment)

4. Anti-ENO3 mouse monoclonal antibody at 1:1000 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: HeLa Whole Cell Lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Rabbit Anti-Mouse IgG3/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:47 kDa.

-Observed band size:47 kDa. (Validation Experiment)

WB,ELISA,IHC-P

WB: 0.05-0.5 μg/mL; ELISA: 0.1-1 μg/mL; IHC-P: 0.1-2.5 μg/mL

Monoclonal

HRP

Unconjugated

2

Recombinant Mouse IgG3 Protein

Mouse