LasR-IN-3 (compound 7f) (4.68-150 μg/mL; 24 h) shows anti-activity against Pseudomonas aeruginosa with MIC and sub-MIC values of 56.25 μg/mL and 14.00 μg/mL [1]. LasR-IN-3 (14.00 μg/mL; 24 h, or 48 h) inhibits biofilm formation, pyocyanin, and rhamnolipids production with inhibition rate of 71.70%, 68.70%, 54.00%, respectively [1]. LasR-IN-3 (56.25 μg/mL, and 338.0 μg/mL; 24 h) is safe to normal cell line (HDFa) [1]. LasR-IN-3 (14.00 μg/mL; 24 h) decreases lasR and rhlR gene expression and inhibits the QS-dependent biofilm formation and virulence factors [1]. RT-PCR [1] Cell Line: Pseudomonas aeruginosa Concentration: 14.00 μg/mL Incubation Time: 24 hours Result: Decreased lasR and rhlR expression by 26% and 16.3%, respectively.