Powder: -20°C for 3 years | In solvent: -80°C for 1 year
GRP78-IN-1 displays interactions with specific residues on the GRP78 protein with a binding energy of -8.07 kcal/mol. This compound possesses potent cytotoxic and anti-proliferative effects on cancer cells, particularly demonstrating promising apoptosis in breast cancer cells and wound healing properties [1].
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 10-14周 | ||
50 mg | ¥ 13,800 | 10-14周 | ||
100 mg | ¥ 17,500 | 10-14周 |
产品描述 | GRP78-IN-1 displays interactions with specific residues on the GRP78 protein with a binding energy of -8.07 kcal/mol. This compound possesses potent cytotoxic and anti-proliferative effects on cancer cells, particularly demonstrating promising apoptosis in breast cancer cells and wound healing properties [1]. |
体外活性 | GRP78-IN-1 (compound 3i) (0.01, 0.1, 1, 10, 100 μM; 48 h) shows the most potent cytotoxic effect (IC 50 s of 2.06, 12.57, 9, 18, 4.9, 2.19, 62.48 μM in MCF-1, PANC-1, HCT-116, PC-3, A549, MDA-MB-231 and FR-2 cells, respectively) [1]. GRP78-IN-1 (1, 2, 4, 6 μM) shows a steady increase in the expression of pro-apoptotic proteins viz. Par-4, apoptotic cascade in BAX and cleaved caspase 9 cells [1]. GRP78-IN-1 (1, 2, 4, 6 μM; 48 h) inhibits the motility of MCF-7 and A549 cells in a dose-dependent manner [1]. Cell Cytotoxicity Assay [1] Cell Line: MCF-1, MDA-MB-231, PANC-1, HCT-116, PC-3, A549, FR-2cells Concentration: 0.01, 0.1, 1, 10, 100 μM Incubation Time: 48 h Result: Showed the most promising cytotoxic effect (IC 50 s is 2.06, 12.57, 9, 18, 4.9, 2.19, 62.48 μM in MCF-1, PANC-1, HCT-116, PC-3, A549, MDA-MB-231 and FR-2 cells, respectively). Western Blot Analysis [1] Cell Line: BCL-2, BAX, cleaved caspase 9, MCF-7, A549 cells Concentration: 1, 2, 4, 6 μM Incubation Time: Result: Showed a steady increase in the expression of pro-apoptotic proteins viz. Par-4, apoptotic cascade in BAX and cleaved caspase 9 cells. |
分子量 | 342.4 |
分子式 | C21H23FO3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
GRP78-IN-1 Inhibitor inhibitor inhibit