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Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP
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产品编号 TMAB-02008H
别名 Immunoglobulin G, Goat Anti-Rabbit IgG H&L (HRP)
Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP 是一种 HRP-conjugated Goat 抗体，靶向 IgG H&L。Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP 可用于 WB,IHC-P,IHC-F,ELISA。
Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP
Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP
还可以产品编号 TMAB-02008H 别名 Immunoglobulin G, Goat Anti-Rabbit IgG H&L (HRP)
Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP 是一种 HRP-conjugated Goat 抗体，靶向 IgG H&L。Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP 可用于 WB,IHC-P,IHC-F,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 175 | 5日内发货 | |
| 1 mL | ¥ 1,190 | 5日内发货 |
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抗原信息
| 产品描述 | Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP can be used in WB,IHC-P,IHC-F,ELISA. |
| 别名 | Immunoglobulin G, Goat Anti-Rabbit IgG H&L (HRP) |
| Ig Type | IgG |
| 交叉反应 | Rabbit |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:300) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:300) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ACE2) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:300) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02008H 1:100) instructionsand DAB staining. 11. Immobilized rabbit IgG (nothing else) at 0.2μg/well can bind Goat Anti-Rabbit IgG H&L/HRP antibody (TMAB-02008H). 12. Sample: Lane 1: SH-SY5Y (Human) Cell Lysate Lane 2: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH at 1/20000 dilution Secondary: Goat Anti-Rabbit IgG H&L-HRP at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD             |
| 应用 | |
| 推荐剂量 | WB: 1:2000-20000; IHC-P: 1:200-1000; IHC-F: 1:200-1000; ELISA: 1:2000-20000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Goat |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Affinity purified by Protein G, nonspecific adsorbed |
| 性状 | Liquid |
| 缓冲液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 浓度 | 2.0 mg/ml |
| 研究背景 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 免疫原 | Native Rabbit IgG |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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