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Goat Anti-Mouse IgG H&L Secondary Antibody-HRP

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Goat Anti-Mouse IgG H&L Secondary Antibody-HRP
产品编号 TMAB-02010H
别名 Immunoglobulin G, Goat Anti-Mouse IgG H&L (HRP)

Goat Anti-Mouse IgG H&L Secondary Antibody-HRP 是一种 HRP-conjugated Goat 抗体，靶向 IgG H&L。Goat Anti-Mouse IgG H&L Secondary Antibody-HRP 可用于 WB,IHC-P,IHC-F,ELISA。

Goat Anti-Mouse IgG H&L Secondary Antibody-HRP

Goat Anti-Mouse IgG H&L Secondary Antibody-HRP

Rating icon 还可以
Goat Anti-Mouse IgG H&L Secondary Antibody-HRP
产品编号 TMAB-02010H 别名 Immunoglobulin G, Goat Anti-Mouse IgG H&L (HRP)

Goat Anti-Mouse IgG H&L Secondary Antibody-HRP 是一种 HRP-conjugated Goat 抗体，靶向 IgG H&L。Goat Anti-Mouse IgG H&L Secondary Antibody-HRP 可用于 WB,IHC-P,IHC-F,ELISA。

规格价格库存数量
100 μL
¥ 175
5日内发货
1 mL
¥ 1,190
5日内发货
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产品介绍

生物活性
产品描述
Goat Anti-Mouse IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. Goat Anti-Mouse IgG H&L Secondary Antibody-HRP can be used in WB,IHC-P,IHC-F,ELISA.
别名Immunoglobulin G, Goat Anti-Mouse IgG H&L (HRP)
Ig Type
IgG
交叉反应
Mouse
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:100) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:100) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:300) instructionsand DAB staining.
4. Immobilized mouse IgG (nothing else) at 0.2μg/well can bind Goat Anti-Mouse IgG H&L/HRP antibody (TMAB-02010H).
5. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:300) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:100) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:300) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:200) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:200) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) (TMAB-02010H 1:200) instructionsand DAB staining.
11. Sample:
Lane 1,3,5 : Rat Cerebrum tissue lysates
Lane 2,4,6 : Human MCF-7 cell lysates
Primary: Anti- GAPDH at 1/5000 dilution
Secondary: Goat Anti-Mouse IgG H&L-HRP (TMAB-02010H) at 1/20000~1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Goat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRPGoat Anti-Mouse IgG H&L Secondary Antibody-HRP
应用
WB
IHC-P
IHC-F
ELISA
推荐剂量
WB: 1:2000-20000; IHC-P: 1:200-1000; IHC-F: 1:200-1000; ELISA: 1:2000-20000
抗体种类
Polyclonal
宿主来源Goat
构建方式Polyclonal Antibody
纯化方式Affinity purified by Protein G, nonspecific adsorbed
性状Liquid
缓冲液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
浓度2.0 mg/ml
研究背景Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
抗原信息
免疫原
Native Mouse IgG
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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