Furamidine dihydrochloride (DB75 dihydrochloride) is a cell-permeable, selective small molecule inhibitor of protein arginine methyltransferase 1 (PRMT1) that also binds preferentially to strings of AT base pair sequences within the minor groove of DNA; it targets the PRMT1 enzyme active site primarily through a competitive mechanism with the protein substrate and is noncompetitive toward the cofactor, ultimately leading to the blockade of cell proliferation in various leukemia cell lines irrespective of their genetic lesions.

PRMT5:166 µM (IC50), Protein arginine methyltransferase 1 (PRMT1):9.4 μM (IC50), PRMT4:>400 µM (IC50), PRMT6:283 µM (IC50)