Donkey Anti-Rabbit IgG H&L Secondary Antibody-AF594 is a AF594-conjugated Donkey antibody targeting IgG H&L. Donkey Anti-Rabbit IgG H&L Secondary Antibody-AF594 can be used in IF,ICC/IF,FCM.

IgG

Rabbit

1. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (TMAB-02007AF594) for 90 minutes, and DAPI for nuclei staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (TMAB-02007AF594) for 90 minutes, and DAPI for nuclei staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (TMAB-02007AF594) for 90 minutes, and DAPI for nuclei staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (TMAB-02007AF594) for 90 minutes, and DAPI for nuclei staining.

IF: 1:100-1000; ICC/IF: 1:100-1000; FCM: 1:100-1000

Polyclonal

Native Rabbit IgG