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Beta-Actin Loading Control Antibody (1W773)
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产品编号 TMAB-00215
Beta-Actin Loading Control Antibody (1W773) 是一种 Mouse 抗体，靶向 Beta-Actin Loading Control。Beta-Actin Loading Control Antibody (1W773) 可用于 WB,IHC-P,IHC-Fr,IF。
Beta-Actin Loading Control Antibody (1W773)
Beta-Actin Loading Control Antibody (1W773)
还可以产品编号 TMAB-00215
Beta-Actin Loading Control Antibody (1W773) 是一种 Mouse 抗体，靶向 Beta-Actin Loading Control。Beta-Actin Loading Control Antibody (1W773) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 670 | 5日内发货 | |
| 100 μL | ¥ 960 | 5日内发货 | |
| 500 μL | ¥ 3,980 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Beta-Actin Loading Control Antibody (1W773) is a Mouse antibody targeting Beta-Actin Loading Control. Beta-Actin Loading Control Antibody (1W773) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 1W773 |
| 交叉反应 | Human,Mouse,Rat (predicted:Chicken,Dog,Rabbit,Hamster,Monkey) |
| 验证活性 | 1. 25 μg total protein per Lane of various lysates probed with beta-Actin monoclonal antibody, unconjugated (TMAB-00215) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (ascites of TMAB-00215) at 1:1500 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-00215) at 1:1000 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. |
| 应用 | |
| 推荐剂量 | WB: 1:500-5000; IHC-P: 1:200-2000; IHC-Fr: 1:200-2000; IF: 1:200-2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting. |
| 免疫原 | Synthetic MAP peptide: human beta-Actin |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Actin,Actin,Beta Actin |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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