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Anti-Wilms tumor/WT1 Antibody (4A565)
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产品编号 TMAH-01253
Anti-Wilms tumor/WT1 Antibody (4A565) 是一种抗体，靶向 Wilms tumor/WT1。Anti-Wilms tumor/WT1 Antibody (4A565) 可用于 ELISA, WB, IHC。
Anti-Wilms tumor/WT1 Antibody (4A565)
Anti-Wilms tumor/WT1 Antibody (4A565)
还可以产品编号 TMAH-01253
Anti-Wilms tumor/WT1 Antibody (4A565) 是一种抗体，靶向 Wilms tumor/WT1。Anti-Wilms tumor/WT1 Antibody (4A565) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Wilms tumor/WT1 Antibody (4A565) is an antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (4A565) can be used in ELISA, WB, IHC. |
| Ig Type | Rabbit IgG |
| 克隆号 | 4A565 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate -All lanes: Wilms Tumor Protein antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 50, 48, 48, 49, 35, 57, 56, 34 kDa -Observed band size: 50 kDa 2. IHC image of TMAH-01253 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Nucleus, nucleolus. Cytoplasm.; [Isoform 1]: Nucleus speckle.; [Isoform 4]: Nucleus, nucleoplasm. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Wilms Tumor Protein |
| 抗原种属 | Human |
| 基因ID | 7490 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Tags & Cell Markers |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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