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Anti-V5 Tag Antibody (9X33)

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产品编号 TMAY-00138

Anti-V5 Tag Antibody (9X33) 是一种 Mouse 抗体,靶向 V5 Tag。Anti-V5 Tag Antibody (9X33) 可用于 ICC/IF。

Anti-V5 Tag Antibody (9X33)

Anti-V5 Tag Antibody (9X33)

Rating icon 还可以
产品编号 TMAY-00138

Anti-V5 Tag Antibody (9X33) 是一种 Mouse 抗体,靶向 V5 Tag。Anti-V5 Tag Antibody (9X33) 可用于 ICC/IF。

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产品介绍

生物活性
产品描述
Anti-V5 Tag Antibody (9X33) is a Mouse antibody targeting V5 Tag. Anti-V5 Tag Antibody (9X33) can be used in ICC/IF.
Ig Type
Monoclonal Mouse IgG1
克隆号
9X33
交叉反应
other
验证活性
Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-V5-Tag monoclonal antibody at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
应用
ICC/IF
推荐剂量
ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5 epitope tag antibody products recognize and detect V5-tagged proteins without a protein-specific antibody or probe in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
抗原种属
other
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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4个月前
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