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Anti-V5 Tag Antibody (9X33)
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还可以产品编号 TMAY-00138
Anti-V5 Tag Antibody (9X33) 是一种 Mouse 抗体，靶向 V5 Tag。Anti-V5 Tag Antibody (9X33) 可用于 ICC/IF。
Anti-V5 Tag Antibody (9X33)
Anti-V5 Tag Antibody (9X33)
一键复制产品信息 还可以产品编号 TMAY-00138
Anti-V5 Tag Antibody (9X33) 是一种 Mouse 抗体，靶向 V5 Tag。Anti-V5 Tag Antibody (9X33) 可用于 ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 780 | 5日内发货 | |
| 100 μL | ¥ 1,580 | 5日内发货 | |
| 500 μL | ¥ 3,980 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-V5 Tag Antibody (9X33) is a Mouse antibody targeting V5 Tag. Anti-V5 Tag Antibody (9X33) can be used in ICC/IF. |
| Ig Type | Mouse IgG1 |
| 克隆号 | 9X33 |
| 交叉反应 | other |
| 特异性 | Recognize N-terminal and C-terminal V5 Tag in fusion proteins |
| 验证活性 | Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-V5-Tag monoclonal antibody at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5 epitope tag antibody products recognize and detect V5-tagged proteins without a protein-specific antibody or probe in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: V5 tag sequence (GKPIPNPLLGLDST). |
| 抗原种属 | other |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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