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Anti-V5 tag Antibody (1D3)

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产品编号 TMAC-04214

别名 V 5 tag

Anti-V5 tag Antibody (1D3) 是一种 Mouse 抗体，靶向 V5 tag。Anti-V5 tag Antibody (1D3) 可用于 IF,IHC,IP,WB。

Anti-V5 tag Antibody (1D3)

Anti-V5 tag Antibody (1D3)

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产品编号 TMAC-04214 别名 V 5 tag

Anti-V5 tag Antibody (1D3) 是一种 Mouse 抗体，靶向 V5 tag。Anti-V5 tag Antibody (1D3) 可用于 IF,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 780
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100 μL
¥ 1,185
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产品介绍

生物活性
产品描述
Anti-V5 tag Antibody (1D3) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (1D3) can be used in IF,IHC,IP,WB.
别名V 5 tag
Ig Type
IgG1
克隆号
1D3
验证活性
1. All lanes: V5-tag mouse monoclonal antibody, A: HEK 293 cell lysate transfected without V5-tag protein, B: HEK 293 cell lysate transfected with V5 -tag protein, C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight), D: IP (PBS + anti- V5 mAb 4℃ overnight), E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight), F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight), G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain.
2. IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5).
3. IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli).
verifiedActivityverifiedActivityverifiedActivity
应用IFIHCIPWB
推荐剂量
WB: 1:3000-10000; IHC: 1:200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
KLH conjugated synthetic peptide: GKPIPNPLLGLDST
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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4个月前
5.0
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