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Anti-USP6 Antibody (1Q774)
还可以产品编号 TMAH-01240
Anti-USP6 Antibody (1Q774) 是一种 Mouse 抗体，靶向 USP6。Anti-USP6 Antibody (1Q774) 可用于 ELISA, WB, IF。
Anti-USP6 Antibody (1Q774)
Anti-USP6 Antibody (1Q774)
还可以产品编号 TMAH-01240
Anti-USP6 Antibody (1Q774) 是一种 Mouse 抗体，靶向 USP6。Anti-USP6 Antibody (1Q774) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-USP6 Antibody (1Q774) is a Mouse antibody targeting USP6. Anti-USP6 Antibody (1Q774) can be used in ELISA, WB, IF. |
| Ig Type | IgG1 |
| 克隆号 | 1Q774 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: USP6 antibody at 1:1000 -Lane 1: MCF-7 whole cell lysate -Lane 2: THP-1 whole cell lysate -Lane 3: SH-SY5Y whole cell lysate -Lane 4: Jurkat whole cell lysate -Lane 5: HEK293 whole cell lysate -Lane 6: Hela whole cell lysate -Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution -Predicted band size: 89, 122, 159 KDa -Observed band size: 89, 122, 159 KDa -Exposure time: 1min 2. Immunofluorescence staining of HeLa cells with TMAH-01240 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 纯度 | >95% |
| 研究背景 | Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human Ubiquitin carboxyl-terminal hydrolase 6 Protein (348-535AA) |
| 抗原种属 | Human |
| 基因ID | 9098 |
| Uniprot ID | |
| 研究领域 | Cell Biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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