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Anti-UCHL3 Antibody (9J995)
还可以产品编号 TMAY-03404
Anti-UCHL3 Antibody (9J995) 是一种 Rabbit 抗体，靶向 UCHL3。Anti-UCHL3 Antibody (9J995) 可用于 WB,IP。
Anti-UCHL3 Antibody (9J995)
Anti-UCHL3 Antibody (9J995)
还可以产品编号 TMAY-03404
Anti-UCHL3 Antibody (9J995) 是一种 Rabbit 抗体，靶向 UCHL3。Anti-UCHL3 Antibody (9J995) 可用于 WB,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-UCHL3 Antibody (9J995) is a Rabbit antibody targeting UCHL3. Anti-UCHL3 Antibody (9J995) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9J995 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa(We are unsure as to the identity of these extra bands.) 2. Mouse UCHL3 was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -2 µL anti-Mouse UCHL3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse UCHL3 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 26 kDa. -Observed band size: 26 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse UCHL3 (rM UCHL3; TMPY-02190; Q9JKB1; Glu2-Ala230). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase that belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Mouse UCHL3 Protein (TMPY-02190) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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