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Anti-TNNI3 Antibody (8F759) 是一种 Rabbit 抗体,靶向 TNNI3。Anti-TNNI3 Antibody (8F759) 可用于 WB,ICC/IF,IHC,IP。
Anti-TNNI3 Antibody (8F759) 是一种 Rabbit 抗体,靶向 TNNI3。Anti-TNNI3 Antibody (8F759) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-TNNI3 Antibody (8F759) is a Rabbit antibody targeting TNNI3. Anti-TNNI3 Antibody (8F759) can be used in WB,ICC/IF,IHC,IP. |
别名 | Troponin I, TNNI3, TNNC1, CMH7 |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of Troponin I on human heart lysates using anti-Troponin I antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Troponin I antibody. Counter stained with hematoxylin. 3. ICC staining Troponin I in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Troponin I in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer composed of two heavy chains (MHC) and four light chains (MLC); it interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin comprises at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites while slow/cardiac Troponin C has a single calcium binding site. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 24 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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