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Anti-Syndecan-4 Antibody (2H831)
还可以产品编号 TMAY-03437
Anti-Syndecan-4 Antibody (2H831) 是一种 Rabbit 抗体，靶向 Syndecan-4。Anti-Syndecan-4 Antibody (2H831) 可用于 IHC-P。
Anti-Syndecan-4 Antibody (2H831)
Anti-Syndecan-4 Antibody (2H831)
还可以产品编号 TMAY-03437
Anti-Syndecan-4 Antibody (2H831) 是一种 Rabbit 抗体，靶向 Syndecan-4。Anti-Syndecan-4 Antibody (2H831) 可用于 IHC-P。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Syndecan-4 Antibody (2H831) is a Rabbit antibody targeting Syndecan-4. Anti-Syndecan-4 Antibody (2H831) can be used in IHC-P. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2H831 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Immunofluorescence staining of SDC4 in mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse SDC4 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Immunochemical staining of mouse SDC4 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). |
| 应用 | IHC-P |
| 推荐剂量 | IHC-P: 1:100-1:500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4 / SDC4 (rM Syndecan-4 / SDC4; TMPY-02392; O35988; Met1-Val146). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with several signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Mouse Syndecan-4 / SDC4 protein (TMPY-02392) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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