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Anti-Synaptophysin/SYP Antibody (1U942)
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产品编号 TMAC-03936
Anti-Synaptophysin/SYP Antibody (1U942) 是一种 Rabbit 抗体，靶向 Synaptophysin/SYP。Anti-Synaptophysin/SYP Antibody (1U942) 可用于 WB,ICC/IF,IHC。
Anti-Synaptophysin/SYP Antibody (1U942)
Anti-Synaptophysin/SYP Antibody (1U942)
还可以产品编号 TMAC-03936
Anti-Synaptophysin/SYP Antibody (1U942) 是一种 Rabbit 抗体，靶向 Synaptophysin/SYP。Anti-Synaptophysin/SYP Antibody (1U942) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Synaptophysin/SYP Antibody (1U942) is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Antibody (1U942) can be used in WB,ICC/IF,IHC. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution. Positive control: Lane 1: Human brain, Lane 2: PC-12. 2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. 5. ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 38 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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