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Anti-STAG2 Antibody (9P946)
还可以产品编号 TMAH-01135
别名 Stromal antigen 2, STAG 2, SCC3 homolog 2, SA2, Cohesin subunit SA-2
Anti-STAG2 Antibody (9P946) 是一种抗体，靶向 STAG2。Anti-STAG2 Antibody (9P946) 可用于 ELISA, WB, IF。
Anti-STAG2 Antibody (9P946)
Anti-STAG2 Antibody (9P946)
还可以产品编号 TMAH-01135 别名 Stromal antigen 2, STAG 2, SCC3 homolog 2, SA2, Cohesin subunit SA-2
Anti-STAG2 Antibody (9P946) 是一种抗体，靶向 STAG2。Anti-STAG2 Antibody (9P946) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-STAG2 Antibody (9P946) is an antibody targeting STAG2. Anti-STAG2 Antibody (9P946) can be used in ELISA, WB, IF. |
| 别名 | Stromal antigen 2, STAG 2, SCC3 homolog 2, SA2, Cohesin subunit SA-2 |
| Ig Type | Rabbit IgG |
| 克隆号 | 9P946 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate -All lanes: SA2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 142, 146 kDa -Observed band size: 142 kDa 2. Immunofluorescence staining of MCF7 Cells with TMAH-01135 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human SA2 |
| 抗原种属 | Human |
| 基因ID | 10735 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cell biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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