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Anti-Sodium Potassium ATPase Antibody (8O42)

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Anti-Sodium Potassium ATPase Antibody (8O42)
产品编号 TMAB-01753

Anti-Sodium Potassium ATPase Antibody (8O42) 是一种 Mouse 抗体，靶向 Sodium Potassium ATPase。Anti-Sodium Potassium ATPase Antibody (8O42) 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-Sodium Potassium ATPase Antibody (8O42)

Anti-Sodium Potassium ATPase Antibody (8O42)

Rating icon 还可以
Anti-Sodium Potassium ATPase Antibody (8O42)
产品编号 TMAB-01753

Anti-Sodium Potassium ATPase Antibody (8O42) 是一种 Mouse 抗体，靶向 Sodium Potassium ATPase。Anti-Sodium Potassium ATPase Antibody (8O42) 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,960
5日内发货
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产品介绍

生物活性
产品描述
Anti-Sodium Potassium ATPase Antibody (8O42) is a Mouse antibody targeting Sodium Potassium ATPase. Anti-Sodium Potassium ATPase Antibody (8O42) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG1,k
克隆号
8O42
交叉反应
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
18. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
19. 25 μg total protein per Lane of various lysates probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (TMAB-01753) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
20. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
21. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
22. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (TMAB-01753) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:2000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cell membrane; Multi-pass membrane protein. Melanosome.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009].
抗原信息
免疫原
Recombinant Protein: human Sodium Potassium ATPase
抗原种属
Human
基因ID
476
Uniprot ID
P05023
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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