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顶部生物活性储存和溶解信息计算器

Anti-SOD2 Antibody (8Z611)

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Anti-SOD2 Antibody (8Z611)
产品编号 TMAY-02405
别名 superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB

Anti-SOD2 Antibody (8Z611) 是一种 Rabbit 抗体，靶向 SOD2。Anti-SOD2 Antibody (8Z611) 可用于 WB,ELISA,IP。

Anti-SOD2 Antibody (8Z611)
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Anti-SOD2 Antibody (8Z611)

Rating icon 还可以
Anti-SOD2 Antibody (8Z611)
产品编号 TMAY-02405 别名 superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB

Anti-SOD2 Antibody (8Z611) 是一种 Rabbit 抗体，靶向 SOD2。Anti-SOD2 Antibody (8Z611) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 2,294
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产品介绍

生物活性
产品描述
Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in WB,ELISA,IP.
别名superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB
Ig Type
Rabbit IgG
克隆号
8Z611
交叉反应
Human
验证活性
1. SOD2 was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-SOD2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-SOD2 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 16 kDa.
-Observed band size: 18 kDa.
2. Anti-SOD2 rabbit monoclonal antibody at 1:10000 dilution.
-Lane A: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:25 kDa.
-Observed band size:25 kDa.
3. Anti-SOD2 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: SOD2 konckout Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:24 kDa.
-Observed band size:24 kDa(Validation Experiment)
应用
WB,ELISA,IP
推荐剂量
WB: 1:2000-1:10000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; TMPY-02044; P04179-1; Lys 25-Lys 222).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Superoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O•-2), the first member in the plethora of mitochondrial reactive oxygen species.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human SOD2 protein (TMPY-02044)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

计算器

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关键词

MVCD-6MVCD 6

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