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Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427)
还可以产品编号 TMAY-02873
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) 是一种 Chimera 抗体，靶向 SARS-CoV/SARS-CoV-2 Nucleocapsid。Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) 可用于 WB,ELISA,IHC-P,ICC/IF。
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427)
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427)
还可以产品编号 TMAY-02873
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) 是一种 Chimera 抗体，靶向 SARS-CoV/SARS-CoV-2 Nucleocapsid。Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) 可用于 WB,ELISA,IHC-P,ICC/IF。
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抗原信息
| 产品描述 | Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) can be used in WB,ELISA,IHC-P,ICC/IF. |
| Ig Type | Monoclonal Mouse (variable region) / human (kappa / IgG1 constant) chimeric antibody |
| 克隆号 | 9E427 |
| 验证活性 | 1. Anti-SARS-CoV / SARS-CoV-2 Nucleocapsid Chimeric monoclonal antibody at 1:2000 dilution. -Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Human IgG Fc/HRP at 1/1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) 2. Immunochemical analysis of SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a HRP-conjugated second step antibody. (Validation Experiment) 3. Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a FITC-conjugated second step antibody. (Validation Experiment) |
| 应用 | WB,ELISA,IHC-P,ICC/IF |
| 推荐剂量 | WB: 1:2000-1:5000; ELISA: 1:5000-1:10000; IHC-P: 1:20-1:100; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Chimera |
| 构建方式 | It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions, and the variable region was from mouse monoclonal antibody (Cat#40143-MM08). The chimeric monoclonal antibody was produced using recombinant antibody technology. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant SARS-CoV Nucleoprotein / NP Protein (TMPY-02870) |
| 抗原种属 | SARS |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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