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Anti-SARS-CoV-2 Nucleocapsid Antibody (3U950) 是一种 Mouse 抗体,靶向 SARS-CoV-2 Nucleocapsid。Anti-SARS-CoV-2 Nucleocapsid Antibody (3U950) 可用于 WB,ELISA,LFA(Cap)。
Anti-SARS-CoV-2 Nucleocapsid Antibody (3U950) 是一种 Mouse 抗体,靶向 SARS-CoV-2 Nucleocapsid。Anti-SARS-CoV-2 Nucleocapsid Antibody (3U950) 可用于 WB,ELISA,LFA(Cap)。
产品描述 | Anti-SARS-CoV-2 Nucleocapsid Antibody (3U950) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (3U950) can be used in WB,ELISA,LFA(Cap). |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 3U950 |
验证活性 | Anti-SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid mouse monoclonal antibody in 1μg/mL. -Sample: Recombinant Protein 10 ng -Lane 1: SARS-COV-2 JN.1/BA.2.86 (Omicron) Nucleoprotein/NP Protein -Lane 2: SARS-COV-2 BQ.1/BQ.1.1 (Omicron) Nucleocapsid Protein -Lane 3: SARS-COV-2 BF.7 (Omicron) Nucleocapsid Protein -Lane 4: SARS-CoV-2 (BA.4) Nucleocapsid Protein -Lane 5: SARS-CoV-2 Nucleocapsid (P80R) Protein -Lane 6: SARS-CoV-2 Nucleocapsid (T205I) Protein -Lane 7: SARS-CoV-2 Nucleocapsid NTD Protein -Lane 8: SARS-CoV-2 Nucleocapsid CTD Protein -Lane 9: SARS-CoV-2 Nucleocapsid-His recombinant Protein -Lane 10: SARS-CoV-2 Nucleocapsid(D3L, R203K, G204R, S235F)-His Recombinant Protein -Lane 11: SARS-CoV-2 B.1.1.529 (Omicron) Nucleocapsid Protein -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
应用 | WB,ELISA,LFA(Cap) |
推荐剂量 | WB: 1:1000-1:10000; ELISA(Cap): 1 mg/mL |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 Nucleocapsid (TMPY-05664; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
偶联 | Unconjugated |
免疫原 | Recombinant SARS-CoV-2 Nucleocapsid Protein (TMPY-05664) |
抗原种属 | SARS-CoV-2 |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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