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顶部生物活性储存和溶解信息计算器

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)

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产品编号 TMAB-01659
别名 SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, Ormicron Nucleocapsid Protein, Ormicron N Protein, Ormicron mutant, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) 是一种 Rabbit 抗体，靶向 SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized。Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) 可用于 WB。

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930)

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产品编号 TMAB-01659 别名 SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, Ormicron Nucleocapsid Protein, Ormicron N Protein, Ormicron mutant, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) 是一种 Rabbit 抗体，靶向 SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized。Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) 可用于 WB。

规格价格库存数量
50 μL
¥ 1,385
5日内发货
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生物活性
产品描述
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) is a Rabbit antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Antibody (6F930) can be used in WB.
别名SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, Ormicron Nucleocapsid Protein, Ormicron N Protein, Ormicron mutant, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein
Ig Type
IgG
克隆号
6F930
交叉反应
SARS-CoV-2 WT,SARS-CoV-2 (D614G),SARS-CoV-2 Omicron and series of mutants
验证活性
1. Sample:
Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng
Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng
Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng
Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng
Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng
Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng
Primary: Rabbit Anti-SARS-CoV-2 N protein (TMAB-01659) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:45.3 kDa
Observed band size:50 kDa
2. Sample:
Lane 1: SARS-CoV-2 N Protein (WT) at 10ng
Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 2ng
Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 2ng
Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46 kDa
Observed band size:46 kDa
3. Sample:
Lane 1: SARS-CoV-2 N Protein (WT) at 10ng
Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 10ng
Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 10ng
Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:46 kDa
Observed band size:46 kDa
4. The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
5. The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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应用WB
推荐剂量
WB: 1:500-2000
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
组织特异性The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease t
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
抗原信息
免疫原
Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein
基因名称
S
蛋白名称
Spike glycoprotein
研究领域
SARS Coronavirus
功能
attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
化学信息
分子量Theoretical: 46 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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