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Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) 是一种 Mouse 抗体,靶向 SARS-CoV-2 (2019-nCoV) Nucleocapsid。Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) 可用于 WB。
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) 是一种 Mouse 抗体,靶向 SARS-CoV-2 (2019-nCoV) Nucleocapsid。Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) is a Mouse antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) can be used in WB. |
Ig Type | IgG1 |
克隆号 | 5C944 |
交叉反应 | (predicted:SARS-CoV-2) |
验证活性 | 1. Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) Primary: Anti-SARS-CoV-2 (2019-nCoV) nucleocapsid (TMAB-01657) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 52 kDa 2. Sample: Lane 1: SARS-CoV-2 N protein at 50ng Lane 2: SARS-CoV-2 N protein at 25ng Lane 3: SARS-CoV-2 N protein at 5ng Lane 4: SARS-CoV-2 N protein at 2ng Lane 5: SARS-CoV-2 N protein at 1ng Lane 6: SARS-CoV-2 N protein at 0.5ng Primary: Anti-SARS-CoV-2 N protein at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 3. Sample: Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng Primary: Mouse Anti-SARS-CoV-2 N protein (TMAB-01657) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:45.3 kDa Observed band size:50 kD |
应用 | |
推荐剂量 | WB: 1:500-2000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
亚细胞定位 | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Protein G purified |
性状 | Liquid |
缓冲液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
研究背景 | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
免疫原 | Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein |
研究领域 | SARS Coronavirus |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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