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Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747)
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产品编号 TMAB-01655
别名 SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) 是一种 Mouse 抗体，靶向 SARS-CoV-2 (2019-nCoV) Nucleocapsid。Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) 可用于 WB。
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747)
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747)
还可以产品编号 TMAB-01655 别名 SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) 是一种 Mouse 抗体，靶向 SARS-CoV-2 (2019-nCoV) Nucleocapsid。Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 2,490 | 5日内发货 |
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| 产品描述 | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) is a Mouse antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) can be used in WB. |
| 别名 | SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus Nucleocapsid Protein, novel coronavirus N Protein, 2019-nCoV Nucleoprotein, 2019-nCoV nucleocapsid protein, 2019-nCoV N Protein, 2019-nCoV N, 2019nCoV N, 2019 ncov N Protein |
| Ig Type | IgG2b |
| 克隆号 | 1Z747 |
| 交叉反应 | SARS-CoV-2 |
| 验证活性 | 1. Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) Primary: Anti-SARS-CoV-2 (2019-nCoV) nucleocapsid (TMAB-01655) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 48 kDa 2. Sample: Lane 1: SARS-CoV-2 N protein at 50ng Lane 2: SARS-CoV-2 N protein at 25ng Lane 3: SARS-CoV-2 N protein at 5ng Lane 4: SARS-CoV-2 N protein at 2ng Lane 5: SARS-CoV-2 N protein at 1ng Lane 6: SARS-CoV-2 N protein at 0.5ng Primary: Anti-SARS-CoV-2 N protein at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 3. Sample: Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng Primary: Mouse Anti-SARS-CoV-2 N protein (TMAB-01655) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:45.3 kDa Observed band size:50 kD |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. |
| 组织特异性 | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease t |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 浓度 | ≥1 mg/mL |
| 研究背景 | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| 免疫原 | Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein |
| 基因名称 | S |
| 蛋白名称 | Spike glycoprotein |
| 研究领域 | SARS Coronavirus |
| 功能 | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. |
| 分子量 | Theoretical: 46 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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