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Anti-SAE1 Antibody (8M685)
还可以产品编号 TMAY-02674
Anti-SAE1 Antibody (8M685) 是一种 Mouse 抗体，靶向 SAE1。Anti-SAE1 Antibody (8M685) 可用于 ELISA,ICC/IF。
Anti-SAE1 Antibody (8M685)
Anti-SAE1 Antibody (8M685)
还可以产品编号 TMAY-02674
Anti-SAE1 Antibody (8M685) 是一种 Mouse 抗体，靶向 SAE1。Anti-SAE1 Antibody (8M685) 可用于 ELISA,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SAE1 Antibody (8M685) is a Mouse antibody targeting SAE1. Anti-SAE1 Antibody (8M685) can be used in ELISA,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 8M685 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of SAE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Y SAE1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus. |
| 应用 | ELISA,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SAE1 (rh SAE1; TMPY-03062; Q9UBE0; Met1-Lys346). The IgG fraction of the cell culture supernatant was purified by Protein A affinity. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human SAE1 protein (TMPY-03062) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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