Anti-S Antibody (9P501)

Name: Anti-S Antibody (9P501)
Brand: TargetMol
SKU: TMAH-01061
Rating: 4.5 (1 reviews)

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产品编号 TMAH-01061

别名 Spike glycoprotein, S glycoprotein, Peplomer protein, E2

Anti-S Antibody (9P501) 是一种抗体，靶向 S。Anti-S Antibody (9P501) 可用于 ELISA, GICA, Neutralising。

Anti-S Antibody (9P501)

还可以

产品编号 TMAH-01061 别名 Spike glycoprotein, S glycoprotein, Peplomer protein, E2

Anti-S Antibody (9P501) 是一种抗体，靶向 S。Anti-S Antibody (9P501) 可用于 ELISA, GICA, Neutralising。

规格	价格	库存	数量
50 μL	¥ 1,525	5日内发货
100 μL	¥ 2,630	5日内发货

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产品描述	Anti-S Antibody (9P501) is an antibody targeting S. Anti-S Antibody (9P501) can be used in ELISA, GICA, Neutralising.
别名	Spike glycoprotein, S glycoprotein, Peplomer protein, E2
Ig Type	Mouse scFv fusion with human IgG1 Fc
克隆号	9P501
交叉反应	Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
验证活性	1. The Binding Activity of SARS-CoV-2-S Antibody with SARS-CoV-2-S Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 μg/ml can bind SARS-CoV-2-S Antibody, the EC50 is 42.83 ng/ml. 2. The Binding Activity of SARS-CoV-2-S Antibody with SARS-CoV-2-S1-RBD Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody, the EC50 is 29.51 ng/ml. 3. In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (TMAH-01061) is clean, the detection limit can be as low as 223.2ng/ml (15.625ng/0.07ml), and the sensitivity is very good. 4. Binding signal of SARS-CoV-2-S1-RBD and ACE2 protein-HRP conjugate was inhibited by S Antibody (TMAH-01061) with the IC50 is 23.32 nM. 5. Binding signal of SARS-CoV-2-S1-RBD and ACE2 protein-HRP conjugate was inhibited by S Antibody (TMAH-01061) with the IC50 is 2.38 μg/ml. 6. ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-S Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-S Antibody (TMAH-01061) is specific for SARS-CoV-2-S1-RBD protein, without any cross-reactivity with MERS-CoV, SARS-CoV, HCoV-OC43 or HCoV-229E. 7. The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/ml can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody. 8. The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/ml can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody. 9. The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/ml can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody. The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/ml can bind S. 10. The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/ml can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody.
应用	ELISA, GICA
推荐剂量	ELISA:1:10000-1:50000; GICA:1:500-1:5000; Neutralising:1:50-1:10000.
抗体种类	Monoclonal
亚细胞定位	Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
研究背景	attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.

偶联与修饰

偶联	Unconjugated
其他可选修饰符	Biotin/FITC/HRP
可提供抗体种类	4

抗原信息

免疫原	Recombinant Protein: Human Novel Coronavirus Spike glycoProtein (S) (16-685aa)
抗原种属	Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Uniprot ID	P0DTC2
研究领域	Microbiology