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Anti-RPRD1B Antibody (6Y357)
还可以产品编号 TMAY-02686
Anti-RPRD1B Antibody (6Y357) 是一种 Mouse 抗体，靶向 RPRD1B。Anti-RPRD1B Antibody (6Y357) 可用于 ICC/IF。
Anti-RPRD1B Antibody (6Y357)
Anti-RPRD1B Antibody (6Y357)
还可以产品编号 TMAY-02686
Anti-RPRD1B Antibody (6Y357) 是一种 Mouse 抗体，靶向 RPRD1B。Anti-RPRD1B Antibody (6Y357) 可用于 ICC/IF。
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生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-RPRD1B Antibody (6Y357) is a Mouse antibody targeting RPRD1B. Anti-RPRD1B Antibody (6Y357) can be used in ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6Y357 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of RPRD1B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human RPRD1B monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus. |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RPRD1B (rh RPRD1B; TMPY-03599; AAH33629.1; Met1-Asp326). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human RPRD1B Protein (TMPY-03599) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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