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Anti-ROCK2 Antibody (8Y884)
还可以产品编号 TMAH-01048
别名 ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1
Anti-ROCK2 Antibody (8Y884) 是一种抗体，靶向 ROCK2。Anti-ROCK2 Antibody (8Y884) 可用于 ELISA, WB, IF。
Anti-ROCK2 Antibody (8Y884)
Anti-ROCK2 Antibody (8Y884)
还可以产品编号 TMAH-01048 别名 ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1
Anti-ROCK2 Antibody (8Y884) 是一种抗体，靶向 ROCK2。Anti-ROCK2 Antibody (8Y884) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ROCK2 Antibody (8Y884) is an antibody targeting ROCK2. Anti-ROCK2 Antibody (8Y884) can be used in ELISA, WB, IF. |
| 别名 | ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 8Y884 |
| 交叉反应 | Human, Mouse, Rat |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate -All lanes: ROCK2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 161 kDa -Observed band size: 161 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-01048 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human ROCK2 |
| 抗原种属 | Human |
| 基因ID | 9475 |
| Uniprot ID | |
| 研究领域 | Cancer, Cell biology, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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