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Anti-RhoA Antibody (5H334)
还可以产品编号 TMAY-02352
Anti-RhoA Antibody (5H334) 是一种 Rabbit 抗体，靶向 RhoA。Anti-RhoA Antibody (5H334) 可用于 ICC/IF。
Anti-RhoA Antibody (5H334)
Anti-RhoA Antibody (5H334)
还可以产品编号 TMAY-02352
Anti-RhoA Antibody (5H334) 是一种 Rabbit 抗体，靶向 RhoA。Anti-RhoA Antibody (5H334) 可用于 ICC/IF。
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抗原信息
| 产品描述 | Anti-RhoA Antibody (5H334) is a Rabbit antibody targeting RhoA. Anti-RhoA Antibody (5H334) can be used in ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5H334 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human RhoA (rh RhoA; TMPY-01958; P61586-1; Met1-Leu193). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human RhoA protein (TMPY-01958) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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