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Anti-RAC1/2/3 Antibody (1M237)
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产品编号 TMAC-03498
别名 TC-25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac-1, p21-Rac1, MIG5
Anti-RAC1/2/3 Antibody (1M237) 是一种 Rabbit 抗体，靶向 RAC1/2/3。Anti-RAC1/2/3 Antibody (1M237) 可用于 WB,ICC/IF,IHC。
Anti-RAC1/2/3 Antibody (1M237)
Anti-RAC1/2/3 Antibody (1M237)
还可以产品编号 TMAC-03498 别名 TC-25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac-1, p21-Rac1, MIG5
Anti-RAC1/2/3 Antibody (1M237) 是一种 Rabbit 抗体，靶向 RAC1/2/3。Anti-RAC1/2/3 Antibody (1M237) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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| 产品描述 | Anti-RAC1/2/3 Antibody (1M237) is a Rabbit antibody targeting RAC1/2/3. Anti-RAC1/2/3 Antibody (1M237) can be used in WB,ICC/IF,IHC. |
| 别名 | TC-25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac-1, p21-Rac1, MIG5 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of RAC1/2/3 on MCF-7 cells lysates using anti-RAC1/2/3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin. 4. ICC staining RAC1/2/3 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining RAC1/2/3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| 分子量 | Theoretical: 21/24 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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