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Anti-PXN Antibody (8Y831)
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产品编号 TMAC-03085
别名 Paxillin alpha, Paxillin, PAXI_HUMAN, PAX1, PAX 1, Paired box protein Pax 1, FLJ23042, FLJ16691
Anti-PXN Antibody (8Y831) 是一种 Rabbit 抗体，靶向 PXN。Anti-PXN Antibody (8Y831) 可用于 WB,ICC/IF,IHC,IP。
Anti-PXN Antibody (8Y831)
Anti-PXN Antibody (8Y831)
还可以产品编号 TMAC-03085 别名 Paxillin alpha, Paxillin, PAXI_HUMAN, PAX1, PAX 1, Paired box protein Pax 1, FLJ23042, FLJ16691
Anti-PXN Antibody (8Y831) 是一种 Rabbit 抗体，靶向 PXN。Anti-PXN Antibody (8Y831) 可用于 WB,ICC/IF,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PXN Antibody (8Y831) is a Rabbit antibody targeting PXN. Anti-PXN Antibody (8Y831) can be used in WB,ICC/IF,IHC,IP. |
| 别名 | Paxillin alpha, Paxillin, PAXI_HUMAN, PAX1, PAX 1, Paired box protein Pax 1, FLJ23042, FLJ16691 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Paxillin on NIH/3T3 cells lysates using anti-Paxillin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kideny tissue using anti-Paxillin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Paxillin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Paxillin antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Paxillin antibody. Counter stained with hematoxylin. 6. ICC staining Paxillin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Paxillin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Paxillin in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-Src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 65 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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