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Anti-PRMT5 Antibody (3P436)
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产品编号 TMAC-03426
别名 SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5
Anti-PRMT5 Antibody (3P436) 是一种 Rabbit 抗体，靶向 PRMT5。Anti-PRMT5 Antibody (3P436) 可用于 WB,ICC/IF,IHC。
Anti-PRMT5 Antibody (3P436)
Anti-PRMT5 Antibody (3P436)
还可以产品编号 TMAC-03426 别名 SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5
Anti-PRMT5 Antibody (3P436) 是一种 Rabbit 抗体，靶向 PRMT5。Anti-PRMT5 Antibody (3P436) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PRMT5 Antibody (3P436) is a Rabbit antibody targeting PRMT5. Anti-PRMT5 Antibody (3P436) can be used in WB,ICC/IF,IHC. |
| 别名 | SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5 |
| Ig Type | IgG |
| 克隆号 | 3P436 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PRMT5 on A431 cell lysates using anti-PRMT5 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 6. ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The formation of the spliceosome includes the assembly of Sm proteins in an ordered manner onto snRNAs. This process is mediated by the survival of a motor neuron (SMN) protein and is enhanced by modification of specific Arginine residues in the Sm proteins to symmetrical dimethylarginines (sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome, a complex comprised of the type II methyltransferase PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation. The gene encoding human PRMT5 maps to chromosome 14q11. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 73 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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