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顶部生物活性储存和溶解信息计算器

Anti-PRKAG3 Antibody (6D439)

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Anti-PRKAG3 Antibody (6D439)
产品编号 TMAC-03423
别名 Protein kinase AMP activated gamma 3 non catalytic subunit, PRKAG 3, AMPKG3, AMPK subunit gamma-3, AMPK gamma3, AMPK gamma 3 chain, AAKG3_HUMAN, 5''-AMP-activated protein kinase subunit gamma-3, 5 AMP activated protein kinase subunit gamma 3

Anti-PRKAG3 Antibody (6D439) 是一种 Mouse 抗体，靶向 PRKAG3。Anti-PRKAG3 Antibody (6D439) 可用于 WB,ICC,IHC。

Anti-PRKAG3 Antibody (6D439)

Anti-PRKAG3 Antibody (6D439)

Rating icon 还可以
Anti-PRKAG3 Antibody (6D439)
产品编号 TMAC-03423 别名 Protein kinase AMP activated gamma 3 non catalytic subunit, PRKAG 3, AMPKG3, AMPK subunit gamma-3, AMPK gamma3, AMPK gamma 3 chain, AAKG3_HUMAN, 5''-AMP-activated protein kinase subunit gamma-3, 5 AMP activated protein kinase subunit gamma 3

Anti-PRKAG3 Antibody (6D439) 是一种 Mouse 抗体，靶向 PRKAG3。Anti-PRKAG3 Antibody (6D439) 可用于 WB,ICC,IHC。

规格价格库存数量
50 μL
¥ 1,985
5日内发货
100 μL
¥ 2,995
5日内发货
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产品介绍

生物活性
产品描述
Anti-PRKAG3 Antibody (6D439) is a Mouse antibody targeting PRKAG3. Anti-PRKAG3 Antibody (6D439) can be used in WB,ICC,IHC.
别名Protein kinase AMP activated gamma 3 non catalytic subunit, PRKAG 3, AMPKG3, AMPK subunit gamma-3, AMPK gamma3, AMPK gamma 3 chain, AAKG3_HUMAN, 5''-AMP-activated protein kinase subunit gamma-3, 5 AMP activated protein kinase subunit gamma 3
交叉反应
Human
验证活性
1. Western blot analysis of PRKAG3 on human PRKAG3 recombinant protein using anti-PRKAG3 antibody at 1/1,000 dilution.
2. Western blot analysis of PRKAG3 on HEK293 (1) and PRKAG3-hIgGFc transfected HEK293 (2) cell lysate using anti-PRKAG3 antibody at 1/1,000 dilution.
3. Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-PRKAG3 antibody. Counter stained with hematoxylin.
4. ICC staining PRKAG3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC
IHC
推荐剂量
WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enz
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q9UGI9
化学信息
分子量Theoretical: 54 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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