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Anti-PRKAG1 Antibody (6O628)
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产品编号 TMAH-01001
别名 AMPKg, AMPK subunit gamma-1, AMPK gamma1, 5'-AMP-activated protein kinase subunit gamma-1
Anti-PRKAG1 Antibody (6O628) 是一种抗体，靶向 PRKAG1。Anti-PRKAG1 Antibody (6O628) 可用于 ELISA, IHC。
Anti-PRKAG1 Antibody (6O628)
Anti-PRKAG1 Antibody (6O628)
还可以产品编号 TMAH-01001 别名 AMPKg, AMPK subunit gamma-1, AMPK gamma1, 5'-AMP-activated protein kinase subunit gamma-1
Anti-PRKAG1 Antibody (6O628) 是一种抗体，靶向 PRKAG1。Anti-PRKAG1 Antibody (6O628) 可用于 ELISA, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PRKAG1 Antibody (6O628) is an antibody targeting PRKAG1. Anti-PRKAG1 Antibody (6O628) can be used in ELISA, IHC. |
| 别名 | AMPKg, AMPK subunit gamma-1, AMPK gamma1, 5'-AMP-activated protein kinase subunit gamma-1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 6O628 |
| 交叉反应 | Human |
| 验证活性 | IHC image of TMAH-01001 diluted at 1:100 and staining in paraffin-embedded human Prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, IHC |
| 推荐剂量 | IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human PRKAG1 |
| 抗原种属 | Human |
| 基因ID | 5571 |
| Uniprot ID | |
| 研究领域 | Cancer, Cardiovascular, Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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