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Anti-PPM1G Antibody (6W278)

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Anti-PPM1G Antibody (6W278)
产品编号 TMAY-01795
别名 protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG

Anti-PPM1G Antibody (6W278) 是一种 Rabbit 抗体,靶向 PPM1G。Anti-PPM1G Antibody (6W278) 可用于 WB,ELISA,IP。

Anti-PPM1G Antibody (6W278)

Anti-PPM1G Antibody (6W278)

Rating icon 还可以
Anti-PPM1G Antibody (6W278)
产品编号 TMAY-01795 别名 protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG

Anti-PPM1G Antibody (6W278) 是一种 Rabbit 抗体,靶向 PPM1G。Anti-PPM1G Antibody (6W278) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 2,279
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产品介绍

生物活性
产品描述
Anti-PPM1G Antibody (6W278) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (6W278) can be used in WB,ELISA,IP.
别名protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG
Ig Type
Rabbit IgG
克隆号
6W278
交叉反应
Human
验证活性
1. Anti-PPM1G rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: 293T Whole Cell Lysate.
-Lane C: Jurkat Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:59 kDa.
-Observed band size:70 kDa.
2. PPM1G was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg 293T Whole Cell Lysate.
-Lane C:0.5 mg Jurkat Whole Cell Lysate.
-2 µL anti-PPM1G rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-PPM1G rabbit monoclonal antibody, at 1:50 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 59 kDa.
-Observed band size: 72 kDa
应用
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human PPM1G (rh PPM1G; TMPY-02197; O15355; Met317-Asp546).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein that belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human PPM1G protein (TMPY-02197)
抗原种属
Human
研究领域
Phosphatases and Regulators
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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