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Anti-PPM1G Antibody (6W278)
还可以产品编号 TMAY-01795
Anti-PPM1G Antibody (6W278) 是一种 Rabbit 抗体，靶向 PPM1G。Anti-PPM1G Antibody (6W278) 可用于 WB,ELISA,IP。
Anti-PPM1G Antibody (6W278)
Anti-PPM1G Antibody (6W278)
还可以产品编号 TMAY-01795
Anti-PPM1G Antibody (6W278) 是一种 Rabbit 抗体，靶向 PPM1G。Anti-PPM1G Antibody (6W278) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PPM1G Antibody (6W278) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (6W278) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6W278 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-PPM1G rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:59 kDa. -Observed band size:70 kDa. 2. PPM1G was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -Lane C:0.5 mg Jurkat Whole Cell Lysate. -2 µL anti-PPM1G rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PPM1G rabbit monoclonal antibody, at 1:50 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 59 kDa. -Observed band size: 72 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human PPM1G (rh PPM1G; TMPY-02197; O15355; Met317-Asp546). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein that belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PPM1G protein (TMPY-02197) |
| 抗原种属 | Human |
| 研究领域 | Phosphatases and Regulators |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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