Anti-Pokemon/ZBTB7A Antibody (7M948) is a Rabbit antibody targeting Pokemon/ZBTB7A. Anti-Pokemon/ZBTB7A Antibody (7M948) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, IP, WB.

IgG

7M948

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A  Monoclonal Antibody, Unconjugated (TMAB-11617) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A  Monoclonal Antibody, Unconjugated (TMAB-11617) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A  Monoclonal Antibody, Unconjugated (TMAB-11617) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A  Monoclonal Antibody, Unconjugated (TMAB-11617) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A  Monoclonal Antibody, Unconjugated (TMAB-11617) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. 25 ug total protein per lane of various lysates (see on figure) probed with Pokemon/ZBTB7A monoclonal antibody, unconjugated (TMAB-11617) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
7. 4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Pokemon/ZBTB7A) monoclonal Antibody, unconjugated (TMAB-11617) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
8. The HT-29 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Pokemon/ZBTB7A antibody (TMAB-11617, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:100-500; IF=1:200-800; IHC-Fr=1:200-800; IHC-P=1:200-800; IP=1:20-50; WB=1:1000-2000

Monoclonal

A synthesized peptide: human ZBTB7A

Human

ZBTB7A

Zinc finger and BTB domain-containing protein 7A

Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.