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Anti-PIK3R1 Antibody (1Z225)
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产品编号 TMAB-01539
Anti-PIK3R1 Antibody (1Z225) 是一种 Mouse 抗体，靶向 PIK3R1。Anti-PIK3R1 Antibody (1Z225) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-PIK3R1 Antibody (1Z225)
Anti-PIK3R1 Antibody (1Z225)
还可以产品编号 TMAB-01539
Anti-PIK3R1 Antibody (1Z225) 是一种 Mouse 抗体，靶向 PIK3R1。Anti-PIK3R1 Antibody (1Z225) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-PIK3R1 Antibody (1Z225) is a Mouse antibody targeting PIK3R1. Anti-PIK3R1 Antibody (1Z225) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 克隆号 | 1Z225 |
| 交叉反应 | Human,Mouse,Rat (predicted:Pig) |
| 验证活性 | 1. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) monoclonal Antibody, Unconjugated (TMAB-01539) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Sample: Lane 1: Jurkat (Human) Cell Lysate at 30 μg Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 3: Cerebrum (Mouse) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Lane 5: Heart (Mouse) Lysate at 40 μg Lane 6: Heart (Rat) Lysate at 40 μg Lane 7: Skeletal muscle (Mouse) Lysate at 40 μg Lane 8: Skeletal muscle (Rat) Lysate at 40 μg Lane 9: Spleen (Mouse) Lysate at 40 μg Lane 10: MOLT4 (Human) Cell Lysate at 30 μg Primary: Anti-PI 3 Kinase p85 alpha (TMAB-01539) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 85 kDa Observed band size: 85 kDa 3. Sample: BRL-3A Cell (Rat) Lysate at 40 μg Primary: Anti-PI3K p85 (TMAB-01539) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI3K p85) Monoclonal Antibody, Unconjugated (TMAB-01539) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PI 3 Kinase p85 alpha) Monoclonal Antibody, Unconjugated (TMAB-01539) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasmic. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
| 免疫原 | Recombinant Protein: mouse PI3K p85 Protein |
| 抗原种属 | Mouse |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Signal transduction,TLR Signaling,Lipid Kinases |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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