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Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

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Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody
产品编号 TMAC-04281
别名 Wee 1 (p-Ser642), Wee 1 (p-S642), p-Wee 1 (Ser642), p-Wee 1 (S642)

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Wee 1 (Ser642)。Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody 可用于 WB。

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody
产品编号 TMAC-04281 别名 Wee 1 (p-Ser642), Wee 1 (p-S642), p-Wee 1 (Ser642), p-Wee 1 (S642)

Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-Wee 1 (Ser642)。Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody 可用于 WB。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,235
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Wee 1 (Ser642). Anti-Phospho-Wee 1 (Ser642) Polyclonal Antibody can be used in WB.
别名Wee 1 (p-Ser642), Wee 1 (p-S642), p-Wee 1 (Ser642), p-Wee 1 (S642)
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody TMAC-04281. The lane on the right is treated with the antigen-specific peptide.
应用
WB
推荐剂量
WB: 1:500-1000
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
性状Liquid
缓冲液Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1
抗原种属
human
Uniprot ID
P30291
化学信息
分子量Actual: 100 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

Wee 1 (pSer642)Wee 1 (pS642)Wee 1 (phospho-Ser642)Wee 1 (phospho-S642)pWee 1 (Ser642)pWee 1 (S642)

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4个月前
5.0
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