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Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-TOB1 (Ser164)。Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody 可用于 WB,IHC。
Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-TOB1 (Ser164)。Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody 可用于 WB,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,230 | 5日内发货 |
产品描述 | Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TOB1 (Ser164). Anti-Phospho-TOB1 (Ser164) Polyclonal Antibody can be used in WB,IHC. |
别名 | TOB1 (p-Ser164), TOB1 (p-S164), p-TOB1 (Ser164), p-TOB1 (S164) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody TMAC-04076. The lane on the left is treated with antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody TMAC-04076 (left) or the same antibody preincubated with blocking peptide (right). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | This gene encodes a member of the tob/btg1 family of proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1 |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 40 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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