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Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738)
还可以产品编号 TMAY-01658
Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) 是一种 Rabbit 抗体，靶向 Phospho-Stat6 (Tyr641)。Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) 可用于 WB。
Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738)
Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738)
还可以产品编号 TMAY-01658
Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) 是一种 Rabbit 抗体，靶向 Phospho-Stat6 (Tyr641)。Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) 可用于 WB。
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产品介绍
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抗原信息
| 产品描述 | Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) is a Rabbit antibody targeting Phospho-Stat6 (Tyr641). Anti-Phospho-Stat6 (Tyr641) Antibody (6Y738) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6Y738 |
| 交叉反应 | Human; Species predicted to react based on 100% sequence homology: Cynomolgus, Bovin, Pig, Chicken |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with IL4 (100 ng/mL, 15min; +), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution (upper) or Anti-STAT6 Antibody, Rabbit PAb at 1:1000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, treated with IL4 (100 ng/mL, 15 min), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb and other brands’ antibodies (Company C) at dilution of 1:1000, 1:10000 and 1:200000. 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with IL4 using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with IL4 or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:2000-1:20000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr641 of the Human Stat6. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is activated by interleukin-4 (IL-4)-induced tyrosine phosphorylation and mediates most of the IL-4-induced gene expression. STAT6 plays a central role in exerting interleukin-4 (IL-4) mediated biological responses and is found to induce the expression of BCL2L1/BCL-XL, which is responsible for the anti-apoptotic activity of IL4. Transcriptional activation by STAT6 requires the interaction with coactivators like p300 and the CREB-binding protein (CBP). NF-κB and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo, which suggests that the direct interaction between Stat6 and NF-κB may provide a basis for synergistic activation of transcription by IL-4 and activators of NF-κB. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Tyr641 of the Human Stat6 |
| 抗原种属 | Human |
| 研究领域 | Apoptosis Transcription Factors and Regulators |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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