Anti-Phospho-Stat3 (Tyr705) Antibody (4J588) is a Rabbit antibody targeting Phospho-Stat3 (Tyr705). Anti-Phospho-Stat3 (Tyr705) Antibody (4J588) can be used in WB.

Monoclonal Rabbit IgG

4J588

Human;

Predicted to React with:Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovin, Pig;

Phospho-Stat3 (Tyr705)

1. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with IFNα (150 ng/mL, 15 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Stat3 (Tyr705) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment)

2. Western blot analysis of extracts from serum-starved Hela, untreated (-) and treated with IFN alpha 2 (150 ng/mL, 15 min) (+) using Phospho-Stat3 (Tyr705) rabbit monoclonal Antibody at 1:1000 dilution or Beta-Tubulin Loading Control Antibody, Mouse Mab at 1:20000 dilution (lower).

3. Western blot analysis of extracts from serum-starved Hela treated with IFNα (150 ng/mL, 15 min), using Phospho-Stat3 (Tyr705) rabbit monoclonal Antibody and other brands’ antibodies (Company C) at dilution of 1:2000, 1:10000 and 1:50000. (Validation Experiment)

WB

WB: 1:500-1:5000

Monoclonal

Unconjugated

A synthetic phosphopeptide corresponding to residues around Tyr705 of the Human Phospho-Stat3

Human

Cancer Drug Targets, Apoptosis Transcription Factors and Regulators